TY - JOUR T1 - Derivation and Characteristics of ES-Like Cells in Chicken Fetal Ovarian AU - Jia, Qing-Hui AU - Juan, Chen AU - Ping, Shen AU - Fu, Zhi-Xin AU - Liu, Zheng-Zhu AU - Li-Xin, D.U. AU - Zhang, Chuan-Sheng AU - Yin, Chun-Guang AU - Geng, Li-Ying AU - Liu, Xie-Rong JO - Journal of Animal and Veterinary Advances VL - 10 IS - 2 SP - 241 EP - 245 PY - 2011 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2011.241.245 UR - https://makhillpublications.co/view-article.php?doi=javaa.2011.241.245 KW - genome KW -Embryonic stem cells KW -n vitro culture KW -comparison KW -fetal chicken ovarian KW -China AB - In the present study, the ES-like cells were derived from chicken fetal ovarian. These cells exhibit features similar to those of chicken EG cells such as typical bird nest morphology, strong reactivity toward periodic acid-Schiff and alkaline phosphatase staining. They could be maintained for more than a month on STO feeder in vitro, expressed the pluripotent markers such as stage specific embryonic antigen-1, stage-specific embryonic antigen-4, tumor rejection antigen-1-60 and tumor rejection antigen-1-81. Furthermore, the ES-like cells could form embryoid bodies and differentiate to several kinds of cells in vitro. Their easy accessibility makes these cells an attractive alternative to chicken ES cells for future functional and genomic studies and comparison with other stem cells. ER -