TY  - JOUR
T1  - Sequence Variation in <I>Perforin-Like Protein 1</I> Gene among Six<I> Toxoplasma gondii</I> Strains
AU - Zhu, X.Q. AU - Lin, R.Q. AU - Xu, M.J. AU - Ren, D. AU - Zhou, Y. AU - Song, H.Q. AU - Yan, H.K. AU - Yuan, Z.G. AU - Zhou, D.H. 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 17
SP  - 2244
EP  - 2247
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2244.2247
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2244.2247
KW  - Perforin-like Protein 1 (PLP1) gene
KW  -genetic variation
KW  -China
KW  -sequence
KW  -Toxoplasma gondii
KW  -toxoplasmosis
AB  - Perforin-like Protein 1 (PLP1) plays an important role in the invasion process of <I>T. gondii</I>. In this study, we examined sequence variation in the <I>PLP1 </I>gene among six <I>T. gondii</I> strains from Guangzhou and Panyu in Guangdong, Suhe in Henan, Huzhu in Qinghai provinces of China, France and the USA, representing different genotypes. The <I>PLP1</I> gene was amplified from the &gt;6 strains by Polymerase Chain Reaction (PCR) and the amplicons were cloned and sequenced. The length of all of the PLP1 sequences was 3453 bp, consistent with that available in GenBank (EF 102772.2). In total, there were 42 (1.22%) variable nucleotide positions among the six <I>PLP1</I> gene sequences and the 22 of which represented transversions. The A+T contents of the sequences was 48.3~48.9%. Intra-specific nucleotide variation was related mainly to changes at the 2nd and 3rd codon positions while fewer changes were detected at the 1st codon position. These results demonstrated that sequence variation in <I>PLP1</I> gene among the six <I>T. gondii </I>strains was low and the <I>PLP1</I> gene may not be an appropriate marker for the studies of genetic variation among <I>T. gondii </I>strains.
ER  -