TY  - JOUR
T1  - Evaluation of Bacteriocin Resistance in <I>Staphylococcus aureus</I> against the Bacteriocin Complex Secreted by <I>Bacillus subtilis</I> LFB112
AU - Zhang, Ri-Jun AU - Han, Bing AU - Yu, Zhan-Qiao AU - Li, Gui-Guan AU - Liu, Bao-Sheng 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 13
SP  - 1743
EP  - 1749
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1743.1749
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1743.1749
KW  - antibiotic alternative
KW  -fatty acid composition
KW  -fitness cost
KW  -Staphylococcus aureus
KW  -Bacteriocin-resistance
KW  -China
AB  - In order to pre-evaluate the p ossibility of bacteriocin resistance 
  when utilizing a bacteriocin complex against pathogenic bacteria, <I>Staphylococcus 
  aureus</I> was used to develop a resistant variant by stepwise method. The spontaneous 
  frequency of <I>S. aureus</I> resistance to bacteriocin complex was about 10<SUP>-5</SUP>. 
  The stability of resistant variant was confirmed by hundred successive transfers 
  without bacteriocin complex stress. The growth rate of resistant variant was 
  similar as that of its original strain. Interestingly in co-culture system, 
  even inoculated with 90% of resistant variant, the resistant variant was cleared 
  out by wild strain in eight consecutive transfers. When treated with either 
  antibiotics or nisin, the resistant variant was more susceptible than the wild 
  strain to all tested antimicrobial agents except chloramphenicol. However, only 
  the tetracycline and chloramphenicol tested groups showed statistically significant 
  increase and decrease of antibacterial potency (p&lt;0.05), respectively. The 
  cellular membrane fatty acid composition of resistant variant was similar to 
  that of wild strain when cultured without bacteriocin complex suggested that 
  the alterations of membrane fatty acid composition in resistant variants might 
  not be the primary defense against bacteriocin. It is proposed that the bacteriocin 
  complex can be used in animal disease preventions and therapeutics as an alternative 
  or complement to traditional antibiotics.
ER  -