TY  - JOUR
T1  - Polymorphism of Booroola <I>FecB</I> Gene in Prolific Individuals from Najdi and Naeimi Sheep Breeds of Saudi Arabia
AU - Abouheif, M.A. AU - Al-Owaimer, A.N. AU - Shafey, T.M. AU - AlShaikh, M.A. AU - Aljumaah, R.S. 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 10
SP  - 1262
EP  - 1264
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1262.1264
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1262.1264
KW  - FecB gene
KW  -prolificacy
KW  -PCR-RFLP method
KW  -Najdi ewes
KW  -Naeimi ewes
KW  -sheep
KW  -Saudi Arabia
AB  - The present investigation was carried out to examine the presence of Booroola FecB mutation gene in 124 selected prolific ewes from 6 flocks representing two Saudi Arabian local sheep breeds by forced PCR-RFLP method. Genomic DNA was extracted from the blood of 69 Najdi and 55 Naeimi matured ewes with litter size varied from 2.01-2.16 lambs per ewe lambing. Digestion of <I>FecB</I> gene 190 base pair with AvaII restriction enzyme resulted in non carrier 190 bp band wild type in all samples which revealed the absence of this mutation in the tested Najdi and Naeimi sheep breeds.
ER  -