TY  - JOUR
T1  - Construction of Lentivirus-Mediated Retinal Pigment Epithelium Cells Stably Expressing Basic Fibroblast Growth Factors
AU - Huang, Shi-Wei AU - Su, Guan-Fang AU - Wu, Hong 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 9
SP  - 1227
EP  - 1231
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1227.1231
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1227.1231
KW  - Retinal pigment epithelium cell
KW  -lentivirus
KW  -basic fibroblast growth factor
AB  - The transplantation of Retinal Pigment Epithelium (RPE) cells was a potential therapeutic strategy in some retinal diseases. To construct RPE cells capable of stably and efficiently expressing basic Fibroblast Growth Factors (bFGF) using a lentivirus system, we performed the current study. The genes of bFGF were cloned into a lentivirus expression vector then the recombinant lentivirus was packaged and harvested. RPE cells were infected and the expression of the bFGF was detected by western blot. The results showed that the lentivirus vector can efficiently transfer genes into RPE cells with long-term expression. Therefore, it is likely to culture a great number of human RPE cells <I>in vitro</I> to provide adequate and safe low-cost donor cells for RPE cells transplantation.
ER  -