TY  - JOUR
T1  - Effect of Cryopretectants on the Growth Capacity Preservation of Mouse and Cattle Skin
AU - Yu-Rong, Qin AU - Feng, Xu AU - Li-Wei, Ren AU - Hai-Yan, Yang AU - Hao, Chen AU - Min, Sun AU - Bi-Chun, LI AU - Zhi-Quan, Tian AU - Guo-Hong, Chen 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 3
SP  - 540
EP  - 546
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.540.546
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.540.546
KW  - China
KW  -fibroblast
KW  -Cryopreservation
KW  -skin tissues
KW  -cattle
KW  -mouse
AB  - The effect of Dimethylsulphoxide (DMSO), Glycerol (GLY) and Propanedo (PROH) cryoprotectants, each at (10, 20 and 30%) concentration on the preservation of mouse and cattle skin tissue, was investigated. And then, the toxicity test was performed by exposing the skin tissue to DMSO, GLY, PROH, EG without freezing. Tissues were checked by capacity explants. The results indicated that mouse skin tissue frozen in 10% GLY and cattle ear tissue frozen in 20% GLY medium with dry-ice yielded significantly (p<0.05) higher percents (33.3 and 71.4%) of fibroblast than tissue frozen in either concentration of GLY or cryoprotectants. In the toxicity test, mouse skin tissues exposed to GLY and equilibrated at 4°C resulted in higher percent of average explants (41.1%) than that was exposed to PRON and DMSO (average explants 38.3 and 36.5%, respectively), while cattle tissues showed higher toxicity, when exposed to PROH and EG and equilibrated at 4°C (average explants 39.9 and 44.2%, respectively). The present study recommended that 10% GLY with dry-ice freezing was the effective cryoprotectant for the mouse skin tissues, while 20% GLY with dry-ice freezing was effective for cattle skin tissues.
ER  -