TY  - JOUR
T1  - Immune Response Produced by <I>Brucella ovis </I>and Mutants in <I>virB10</I> 
  and <I>virB11</I> in Murine Model
AU - , Marisela Leal-Hernandez AU - , Enrique Salas Tellez AU - , Alma Nunez Del Arco AU - , Rodolfo A. Ugalde AU - , Victor R. Tenorio-Gutierrez AU - , Rigoberto Hernandez-Castro AU - , Francisco Suarez Guemes AU - , Efren Diaz-Aparicio 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 5
SP  - 928
EP  - 934
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.928.934
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.928.934
KW  - Brucella ovis
KW  -immune response
KW  -cytokines
KW  -antibodies
KW  -mouse
KW  -virB mutants
AB  - The aim of this research was to evaluate the humoral and cellular response, produced in mice inoculated with <I>Brucella ovis</I> and mutants in <I>virB10 and virB11, </I>measuring IgG1, IgG2a, IgG2b and IgM levels and cytokines production IL-2, IL-4 e IFN-&#947;, in lymphocyte culture. Mice of 6-weeks-old Balb/c were immunized intraperitoneally with each strain of <I>B. ovis </I>and the mutants with 3x10<SUP>13</SUP> CFU mL<SUP>-1</SUP> in PBS. For the cellular response, they were injected 2 times. In the humoral response the predominant antibodies were IgG2b and IgM in the different strains. The cytokine production with the wild type were indicative of Th2 and low response of IFN-&#947; and IL-2, that is indicative of an answer Th1 type characteristic of intracellular bacteria. The Reo 198 strain, showed Th1 type response. The mutant &#916;<I>virB11</I> only presented response to IFN-&#947; 24 h that decreases after 48 h and with the mutant <I>virB10</I>:: Gm the shown levels of the different cytokine were low, this is indicative that the genes <I>virB10</I> and <I>virB11 </I>of<I> B. ovis</I> need are complete to induce a suitable immune response. In the virulence in mice only recovered the Reo and wild type strains until 1 week postinoculation and the mutants can recover until 4 and 5 weeks postinoculation. One concludes the immune response by the different strains of <I>B. ovis </I>was moderate and can be attributed to that the murine model not was the more recommended to evaluate to cellular response would presumably have been specific to specie by the poorly or inability response showed when were mice inoculated with the different strains of <I>B. ovis</I>.
ER  -