TY  - JOUR
T1  - Differential Diagnosis of Antibody to Classical Swine Fever Virus Field Strain by ELISA with Recombinant E2 Proteins of Various Group CSFV
AU - , Qiu Chang-Qing AU - , Hu Hui AU - , Cao Xiao-An AU - , Zhou Ji-Zhang AU - , Lin Guo-Zhen 
JO  - Journal of Animal and Veterinary Advances
VL  - 7
IS  - 8
SP  - 972
EP  - 980
PY  - 2008
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2008.972.980
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2008.972.980
KW  - CSFV
KW  -recombinant E2 protein
KW  -indirect ELISA
KW  -differential diagnosis
AB  - In this study, an indirect Enzyme-Linked Immunosorbent Assay (ELISA) method for measuring antibodies to Classical Swine Fever Virus (CSFV) was established using recombinant E2 proteins of CSFV C and LT strains, respectively. The optimum dilutions of the E2 antigens and serum were 1:160 and 1:320, respectively. Coating concentration of the E2 antigen was 11.7 µg mL <SUP>1</SUP>; the optimal concentration of enzyme-labeled antibody was 1:800; the optimal reaction times of the first antibody and secondary antibody were 1 h and 45 min, respectively. The tests showed that the E2 antigens were not reacted with both anti-BVD antibody and antibodies against other pathogens which are able to induce abortion of pregnant pigs and death of young piglets.  A  critical  value  of  the<I> </I> method was 0.3503, namely OD of the tested serum  0.35, it was positive for CSF; &lt;0.35, it was negative for CSF. It was found that serum specimens from CSFV LT field strain affected pigs with being diluted to 1:1280 or more could be distinguished by the method, which showed positive in LT strain-E2 ELISA but negative in C strain-E2 ELISA.
ER  -