TY  - JOUR
T1  - Application of Polymerase Chain Reaction Assay in Detection of <I>Leptospira icterohaemorrhagiae</I> in Experimentally Infected Mice
AU - , O. Sharina AU - , S. Khairani-bejo AU - , A.R. Bahaman 
JO  - Journal of Animal and Veterinary Advances
VL  - 6
IS  - 10
SP  - 1197
EP  - 1198
PY  - 2007
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2007.1197.1198
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2007.1197.1198
KW  - Blood
KW  -kidney
KW  -PCR
KW  -Leptospira icterohaemorrhagiae
AB  - A total of 24 mice were inoculated with 1×10<SUP>7</SUP> of <I>Leptospira icterohaemorrhagiae.</I> Four mice as negative control were not inoculated. Four inoculated mice were sacrificed at each sampling day on day 1, 3, 7, 10, 14 and 21 post inoculation. Blood and kidney samples were collected and inoculated into JS medium for isolation of <I>L. icterohaemorrhagiae. </I>Polymerase Chain Reaction (PCR) assay was performed to detect the present of the organisms in blood and kidneys. All culture samples were negative for <I>L. icterohaemorrhagiae</I>. However, one (1/24) blood sample was positive by PCR. It is apparent that detection of leptospires in blood with PCR is a promising approach for early diagnosis of leptospirosis.
ER  -