TY  - JOUR
T1  - The Use of Nuclear Transfer Procedure for Evaluation of Abattoir Derived Bovine Oocyte
AU - , Mohamed Sayed Mohamed Nour AU - , M. Nagano AU - , S. Katagiri AU - , Y. Takahashi 
JO  - Journal of Animal and Veterinary Advances
VL  - 3
IS  - 12
SP  - 800
EP  - 803
PY  - 2004
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2004.800.803
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2004.800.803
KW  - 
AB  - Using nuclear transfer procedure, this study was conducted to investigate the effect of oocyte quality on the in vitro development of bovine embryos. Immature oocytes from slaughterhouse-collected ovaries were classified into good and poor quality based on their morphological appearance. After maturation culture, the good quality group showed a higher percentage of oocytes with first polar body than the poor one. The oocytes with first polar body from both groups were enucleated, activated, and fused with disaggregated donor blastomeres of 32-cell-stage embryos. Enucleation rates of the oocytes with the first polar body were the same for both groups. After nuclear transfer, no significant difference was detected in fusion and cleavage rates between the 2 groups. However, the embryos reconstituted from good quality oocytes showed significantly higher developmental rate to the blastocyst stage and blastocyst cell number than those from the poor ones. These results indicate the necessity of strict selection of oocytes with good morphology as recipient cytoplast for nuclear transfer.
ER  -