@article{MAKHILLJMG202113128913,
    title = {A Consensus Sequence among Natural Transmembrane Linkers for Single-Polypeptide Chain
(SC) Connection of Two G-Protein-Coupled Receptors in Tandem},
    journal = {Journal of Molecular Genetics},
    volume = {13},
    number = {1},
    pages = {1-45},
    year = {2021},
    issn = {2070-4267},
    doi = {jmolgene.2021.1.45},
    url = {https://makhillpublications.co/view-article.php?issn=2070-4267&doi=jmolgene.2021.1.45},
    author = {Toshio,Takashi,Dasiel Oscar,Haruo and},
    keywords = {Transmembrane single polypeptide chain linker,oligomerization,adenosine A2A receptor,dopamine D2 receptor; receptor allostery,fusion protein,striatum,supramolecular protein assembly,consensus sequence of
natural scGPCR TM linker},
    abstract = {A transmembrane (TM) single-polypeptide
chain (sc) linker that can connect any two class A Gprotein-
coupled receptors (GPCRs) in tandem as a genefusion
strategy has been demonstrated. Here, we report
that a natural TM linker for sc exists that can connect two
GPCRs in tandem. <i>In silico</i> analysis indicated that the
central intervening portion (amino acid residues
311~416) of <i>Exaiptasia diaphana</i> (LOC110241027)
GPCR fusion protein contains a single probable TM
domain and that a consensus sequence, T(I/A/P) (A/S)
(L/N) (I/W/L) (I/A/V) GL (L/G) (A/T) (S/L/G) (I/L) is
conserved between this TM and previously characterized
TM-linkers deriving from the type II TM proteins with a
cytoplasmic N-ter segment, single TM and extracellular
C-ter tail, <i>Caenorhabditis elegans odr4</i> and human CD23.
In addition, to the invertebrate TM-linker, it was
found that in the central intervening region of
tropical bird <i>Opisthocomus hoazin</i> protein
LOC104327099 (XP_009930279.1), TM-linker contains
the consensus sequence. Here, we directed for the first
time, our attention to the natural TM sc linker as a rare
evolutionary event among all mammalian and avian
proteins.}
    }