@article{MAKHILLAJ20138520605,
    title = {The Effect of C-Jun on Leydig Cells Proliferation of Elemental Status in Rongchang Piglets},
    journal = {Agricultural Journal},
    volume = {8},
    number = {5},
    pages = {256-261},
    year = {2013},
    issn = {1816-9155},
    doi = {aj.2013.256.261},
    url = {https://makhillpublications.co/view-article.php?issn=1816-9155&doi=aj.2013.256.261},
    author = {Xiaobo,Rong,Jiao,Zhiwan,Lijuan,Libin,Yinzhu,Boqing,Huihao,Maosen,Jing,Qun and},
    keywords = {C-jun,piglet,leydig cells,proliferate,China},
    abstract = {Leydig cells were isolated from the testis of 3 week old Rongchang piglets. Its activity, purity and function were qualified before being cultured <I>in vitro</I>. Then, they were respectively treated with 0.125, 0.25, 0.5, 1 and 2 &#956;mol L<SUP>-1</SUP> c-jun ASODNs and the expression of c-jun mRNA were detected by FQ-PCR. To study the effect of c-jun on proliferation of elemental status leydig cells in piglets, the leydig cells had been treatedwith c-jun ASODNs and the growth inhibition ratio of leydig cells were detected by MTT method and cell cycle phase distribution were examined by flow cytometer analysis. The FQ-PCR assays results indicated that c-jun mRNA expression decreased with the rise of c-jun ASODNs concentration. Only 0.125 &#956;mol L<SUP>-1</SUP> c-jun ASODNs could reduce the expression of <I>c-jun</I> gene significantly (p&lt;0.01). And the expression no longer went down when the concentration was up to 1 &#956;mol L<SUP>-1</SUP>. Through MTT analysis, it is found that c-junASODNs could inhibit the growth of leydig cells significantly (p&lt;0.01). There is a positive correlation between c-jun ASODNs concentration and growth inhibition ratio of leydig cells (r = 0.8966).}
    }