@article{MAKHILLRJP20115512464,
    title = {<I>In vivo</I> and <I>In vitro</I> Effect of Sulfamerazine on Hepatic Mixed Function Oxidase in Rats},
    journal = {Research Journal of Pharmacology},
    volume = {5},
    number = {5},
    pages = {53-58},
    year = {2011},
    issn = {1815-9362},
    doi = {rjpharm.2011.53.58},
    url = {https://makhillpublications.co/view-article.php?issn=1815-9362&doi=rjpharm.2011.53.58},
    author = {P.B. and},
    keywords = {Aminopyrine N-demethylase,aniline hydroxylase,cytochrome P-450,mixed function oxidase,sodium,sulfamerazine},
    abstract = {Sulfamerazine (SMR) administration (i.p., 3 days) of different doses to adult male rats showed significant decrease in electron transport components and drug metabolizing enzymes. In longer duration study, 100 mg SMR kg<SUP>-1</SUP> caused significant decreases in cytochrome P-450 and activities of aminopyrine N-demethylase and aniline hydroxylase. In inhibitory dose of 100 mg SMR kg<SUP>-1</SUP> was selected for dosing young male, old male and adult female rats. Sulfamerazine administration to young, old male and female rats resulted in a significant decrease in electron transport component and drug metabolizing activities at 100 mg SMR kg<SUP>-1</SUP> dose level. All other parameters were unchanged. Sulfamerazine resulted in uncompetitive type of inhibition (Ki = 3.0 mM) of aminopyrine N-demethylase <I>in vitro</I>. Sulfamerazine destructed the spectral and catalytic activity of cytochrome P-450. The studies suggest that SMR is a substrate of the mixed function oxidase system and inhibition is dependent on dosage, age and sex of the animals.}
    }