@article{MAKHILLRJBS20094910994, title = {Antibacterial Effect of Synthesized Silver/Montmorillonite Nanocomposites by UV-Irradiation Method}, journal = {Research Journal of Biological Sciences}, volume = {4}, number = {9}, pages = {1056-1060}, year = {2009}, issn = {1815-8846}, doi = {rjbsci.2009.1056.1060}, url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2009.1056.1060}, author = {Mansor Bin,Majid,Kamyar,Abdul Halim,Nor Azowa,Azizah Abdul and}, keywords = {Silver nanoparticles,montmorillonite,nanocomposite,UV-irradiation,antibacterial activity,muller-hinton agar}, abstract = {Silver nanoparticles (Ag-NPs) were successfully synthesized into the interlayer space of Montmorillonite (MMT) by UV-irradiation method. AgNO3 and Montmorillonite (MMT) were used as a silver precursor and solid support, respectively. The properties of Ag/MMT nanocomposites were studied as a function of UV-irradiation time. UV-irradiation disintegrated the Ag NPs into smaller size until a relatively stable size and size distribution were achieved. The UV-vis spectra of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks increased with increasing UV-irradiation time. The synthesized Ag/MMT nanocomposites under longer UV irradiation time are very stable over a long time in aqueous solution without any sign of agglomeration or precipitants. The crystalline structure of the Ag-NPs and basal spacing of MMT and Ag/MMT were also studied by Powder X-Ray Diffraction (PXRD). The antibacterial activity of Ag-NPs was investigated against Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae) and Gram-positive bacteria (Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA)) by disk diffusion method using Muller-Hinton Agar (MHA) at different sizes of Ag-NPs. All of synthesized Ag-NPs were found to have high antibacterial activity. These results showed that Ag-NPs can be useful in different biomedical research and applications such as diagnostic and surgical devices, contrast agents, drug delivery vehicles and physical therapy applications.} }