@article{MAKHILLRJBS20083110506, title = {The Influence of Cold Acclimation on Proline, Malondialdehyde (MDA), Total Protein and Pigments Contents in Soybean (Glycine max) Seedlings}, journal = {Research Journal of Biological Sciences}, volume = {3}, number = {1}, pages = {74-79}, year = {2008}, issn = {1815-8846}, doi = {rjbsci.2008.74.79}, url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2008.74.79}, author = {Leila Zeinali Yadeghari,Reza Heidari and}, keywords = {Acclimation,chilling,chlorophyll a and chlorophyll b,carotenoids,lipid peroxidation,proline,protein}, abstract = {Low temperature damage is a common problem for early-planted soybean, because it is a tropical plant and is sensitive to low temperatures. In this research plant`s response to cold acclimation and nonacclimation was investigated in soybean (Glycine max). Seedlings were exposed to 15°C (cold-acclimated) or 25°C (nonacclimated) for 24 h, under 250 µmol m 2 s 1 Photosyntetically Active Radiation (PAR). Then, all plants were exposed to chilling temperature at 4°C for 24 h and allowed to recover at 25°C for 24 h. Physiological responses to chilling, including, MDA, proline, chlorophyll a and b and carotenoids and total protein contents were measured in soybean to identify mechanisms of chilling tolerance. Relative water content showed that cold-acclimated plants were less affected by chilling compared to nonacclimated plants. Cold-acclimated plants also recovered faster from chilling injury than nonacclimated plants.} }