@article{MAKHILLJFT20141218325,
title = {Survey and Determination of Mycotoxins in Wheat from Iran by LC/Fluorescence
Using Immunoaffinity Column Cleanup},
journal = {Journal of Food Technology},
volume = {12},
number = {1},
pages = {7-10},
year = {2014},
issn = {1684-8462},
doi = {jftech.2014.7.10},
url = {https://makhillpublications.co/view-article.php?issn=1684-8462&doi=jftech.2014.7.10},
author = {Javad,Hamed Reza and},
keywords = {HPLC,aflatoxin,ochratoxin A,zearalenone,wheat},
abstract = {Mycotoxins are small, toxic chemical products formed as secondary
metabolites by a few fungal spices that readily colonise crops and contaminate
them with toxins in the field or after harvest. In this study, a Liquid Chromatographic
Method was developed for the simultaneous determination of Aflatoxins (AFs)
(B1, B2, G1, G2), Ochratoxin A (OTA)
and Zearalenone (ZEA) toxins in wheat. For this purpose, a total of 231 wheat
samples were analyzed. Mycotoxins were extracted and purified from the samples
using Immunoaffinity (IAC) columns. The 2.67 and 2.21% of examined wheat samples
contained AFB1 and OTA. The method was based on the extraction of
AFs, OTA and ZEA finely ground wheat sample with 80% methanol and 60% acetonitrile
solution, respectively. The 2.60 and 2.16% of examined wheat samples contained
AFB1 and OTA. LOD was 0.27, 0.04, 0.23, 0.04, 1.18 and 21.56 for
AFB1, AFB2, AFG1, AFG2, OTA and
ZEA, respectively. Considering these results these special commodities should
not be a health concern for these collectives, however, special attention should
be focused on imported foods distributed in special retail shops.}
}