@article{MAKHILLJFT2003137821, title = {Biosynthesis of Lipase by Rhizopus oligosporous ISUUV-16 using Agricultural By-products as Substrate}, journal = {Journal of Food Technology}, volume = {1}, number = {3}, pages = {111-114}, year = {2003}, issn = {1684-8462}, doi = {jftech.2003.111.114}, url = {https://makhillpublications.co/view-article.php?issn=1684-8462&doi=jftech.2003.111.114}, author = {Umar Farooq Awan,Shazia Mirza,Kiran Shafiq,Sikander Ali and}, keywords = {}, abstract = {The present study is concerned with the biosynthesis of lipase by Rhizopus oligosporous ISUUV-16 through solid-state fermentation. The optimal substrate for the biosynthesis of lipase was almond meal at the level of 10 g per 250 ml Erlenmeyer flask. The optimal lipase production was obtained when incubated at 300C, for 48 h. Maximal lipase activity of 54 Ug-1 was reached at 1 % level of inoculum. Water was used as diluent for enzyme extraction from fermented mash. Substrate to diluent ratio was kept at 1:0.7.} }