@article{MAKHILLJAVA20131234021,
    title = {ERK MAP-Kinase is Involved in the B7-H1 Expression Induced by Etoposide In Retinoblastoma Cells},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {3},
    pages = {369-376},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.369.376},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.369.376},
    author = {Zhen,Junping,Anhuai,Linying,Zhigang and},
    keywords = {mitogen-activated protein kinases,etoposide,B7-H1,Retinoblastoma,small interfering RNA},
    abstract = {The effect of etoposide on B7-H1 expression was determined by the Reverse-Transcription Polymerase Chain Re-action (RT-PCR), real-time PCR and flow cytometry analysis in Y79 cells. Then, the involvement of Mitogen-Activated Protein Kinases (MAPKs) signal pathways were tested by Western blotting and signal transduction inhibitor assays. Furthmore, specific small interfering RNA (siRNA) targeting B7-H1 was transfected into Y79 retinoblastoma cells using liposome. Silencing of B7-H1 expression was measured by RT-PCR and Western blotting assays. Etoposide increased B7-H1 mRNA and protein levels in Y79 cells. The effect of etoposide on B7-H1 expression peaked at the concentration of 5 &#956;g mL<SUP>-1</SUP> as confirmed by RT-PCR, real-time PCR and flow cytometry assays (p&lt;0.01). The phosphorylation status of Extracellular Signal-Regulated Kinase (ERK), c-Jun N-terminal Kinase (JNK) were constitutively activated by etoposide and MEK inhibitor simultaneously reduced the expression of B7-H1 induced by etoposide (p&lt;0.01). B7-H1 siRNA significantly silenced B7-H1 expression in Y79 cells as confirmed by RT-PCR and Western blotting assays (p&lt;0.01).}
    }