@article{MAKHILLJAVA201211203816, title = {Characterization of β-Actin Promoter of Leuciscus merzbacheri}, journal = {Journal of Animal and Veterinary Advances}, volume = {11}, number = {20}, pages = {3849-3854}, year = {2012}, issn = {1680-5593}, doi = {javaa.2012.3849.3854}, url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.3849.3854}, author = {Shiwei,Wenge,Chuangfu,Yuanzhi,Yan,Xudong and}, keywords = {Leuciscus merzbacheri,promoter analysis,B-actin gene,green fluorescent protein,China}, abstract = {Leuciscus merzbacheri is a unique vulnerable indigenous fish which is only distributed in the Junggar Basin, Xinjiang. In this study, researchers cloned two L. merzbacheri β-actin promoter fragments of different length: SZ11 and SZ21 and analyzed their structural features. The mammalian expression vector pEGFP-N1 was used to construct eukaryotic expression vectors β1 pEGFP-N1-AFP III and β2 pEGFP-N1-AFP III in which fish type III antifreeze protein gene was used as the structural gene. The results showed that the cloned two fragments of β-actin promoters had the ability to drive the expression of the green fluorescent protein gene in BHK-21 cells. These data suggest that the vectors researchers constructed based on β-actin promoter could be exploited as all fish recombinant eukaryotic expression vector.} }