@article{MAKHILLJAVA201211103446, title = {Polymorphism Analysis on Intron 3 of the GH Gene in Chinese Donkey}, journal = {Journal of Animal and Veterinary Advances}, volume = {11}, number = {10}, pages = {1705-1707}, year = {2012}, issn = {1680-5593}, doi = {javaa.2012.1705.1707}, url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1705.1707}, author = {Yongmei,Wenjin and}, keywords = {Donkey,GH gene,intron 3,polymorphism,PCR-SSCP,China}, abstract = {This experiment was conducted to study the polymorphism on intron 3 of GH gene in donkey in China. The polymorphism was analysised by PCR-SSCP in the 10 donkey breeds (JN, GL, GZ, DZ, JM, BY, QY, LZ, TH and XJ) by PCR-SSCP. One SNPs was found in the sequence of GH gene through the sequencing analysis of the two homozygous genes AA and BB, the mutation at 100th site (G→T) of the third intron of donkey GH gene was showed. Three genotypes AA, AB and BB were detected in 10 donkey breeds and the allele A was the predominant allele. All the breeds were in Hardy-Weinberg equilibrium at this polymorphic site (p>0.05). The results confirmed that there were polymorphisms in the intron 3 of GH gene.} }