@article{MAKHILLJAVA201110203003, title = {Identification of One Putative D7 Related Gene Specifically Expressed in Female Anopheles anthropophagus}, journal = {Journal of Animal and Veterinary Advances}, volume = {10}, number = {20}, pages = {2618-2623}, year = {2011}, issn = {1680-5593}, doi = {javaa.2011.2618.2623}, url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.2618.2623}, author = {R.Q.,Y.R.,Y.G.,R.L.,X.Q.,X.H.,C.Y.,S.T.,Y.J. and}, keywords = {sequence analysis,salivary protein,Northern blot,RACE,RT-PCR,Anopheles anthropophagus}, abstract = {Anopheles anthropophagus is an important mosquito vector transmitting malaria and Brugia malayi. Due to differences in feeding behaviors between the female and male adult An. anthropophagus, female An. anthropophagus plays a more important role in transmitting pathogens than male ones. The present study identified a gene specifically expressed in female An. anthropophagus from a subtractive cDNA library for female An. anthropophagus and its full-length cDNA was amplified by 3' and 5' Rapid Amplification of cDNA Ends (RACE) technique. This gene was named fsyz and further analyzed by using Northern blot and bio-informatics analyses. It was shown that the fsyz could encode a D7 related salivary protein and was female-specifically expressed and not characterized previously worth further studies.} }