@article{MAKHILLJAVA2008781183,
    title = {Differential Diagnosis of Antibody to Classical Swine Fever Virus Field Strain by ELISA with Recombinant E2 Proteins of Various Group CSFV},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {7},
    number = {8},
    pages = {972-980},
    year = {2008},
    issn = {1680-5593},
    doi = {javaa.2008.972.980},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2008.972.980},
    author = {Qiu Chang-Qing,Hu Hui,Cao Xiao-An,Zhou Ji-Zhang and},
    keywords = {CSFV,recombinant E2 protein,indirect ELISA,differential diagnosis},
    abstract = {In this study, an indirect Enzyme-Linked Immunosorbent Assay (ELISA) method for measuring antibodies to Classical Swine Fever Virus (CSFV) was established using recombinant E2 proteins of CSFV C and LT strains, respectively. The optimum dilutions of the E2 antigens and serum were 1:160 and 1:320, respectively. Coating concentration of the E2 antigen was 11.7 µg mL <SUP>1</SUP>; the optimal concentration of enzyme-labeled antibody was 1:800; the optimal reaction times of the first antibody and secondary antibody were 1 h and 45 min, respectively. The tests showed that the E2 antigens were not reacted with both anti-BVD antibody and antibodies against other pathogens which are able to induce abortion of pregnant pigs and death of young piglets.  A  critical  value  of  the<I> </I> method was 0.3503, namely OD of the tested serum  0.35, it was positive for CSF; &lt;0.35, it was negative for CSF. It was found that serum specimens from CSFV LT field strain affected pigs with being diluted to 1:1280 or more could be distinguished by the method, which showed positive in LT strain-E2 ELISA but negative in C strain-E2 ELISA.}
    }