@article{MAKHILLJAVA20065111474,
    title = {Cytotoxic Activity in Fermenter Culture Supernatants of<I> Corynebacterium seudotuberculosis</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {5},
    number = {11},
    pages = {939-942},
    year = {2006},
    issn = {1680-5593},
    doi = {javaa.2006.939.942},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2006.939.942},
    author = {K.M. Suleima,H. Boehnel,S.H. Babiker and},
    keywords = {Corynebacterium pseudotuberculosis,fermenter,cytotoxicity,cell lines},
    abstract = {Fermenter Culture supernatants of <I>Corynebacterium</I> <I>pseudotuberculosis</I> strain 3450 NCTC grown in a continuous culture were tested for cytotoxicity in 7 cell lines. The supernatants were toxic to EBL, MDCK, NBL, CHO-K1, 3T3, BHK and Vero cells. The cytotoxic activity was used as an assay method to determine the optimal incubation temperature and pH for cytotoxin production in fermenter. Fermenter culture supernatants of a reference strain <I>C. pseudotuberculosis</I> and three field isolates were compared for their cytotoxicity to BHK cells. Two of the field isolates were non-cytotoxic to BHK cells, one isolate had weak cytotoxicity and the reference strain was the most cytotoxic. SDS-PAGE of fermenter culture supernatants of the 4 strains revealed 35 to 39 bands with molecular weights ranging from 24 to 149 KDa.}
    }